Caspian Journal of Internal Medicine
1
Caspian J Intern Med
Medical Sciences
http://caspjim.com
1
admin
2008-6164
2008-6172
8
10.22088/cjim
14
8888
13
en
jalali
1396
4
1
gregorian
2017
7
1
8
4
online
1
fulltext
en
Molecular characterization of Staphylococcus aureus strains isolated among hospital staff nasal carriers of Babol, Iran
Microbiology
Microbiology
Original Article
Original Article
<p><span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#0000FF;"><strong><em>Background: </em></strong></span><em>Staphylococcus aureus</em> (s. aureus) nasal carriers, particularly the healthcare staff can be considered as a potential source for the spread of resistant strains. The aim of this study was to determine the molecular characterization of S. aureus strains isolated among the staff nasal carriers in one of the teaching hospitals in Babol.<br>
<span style="color:#0000FF;"><strong><em>Methods: </em></strong></span>A total of 120 nasal swabs were taken from the staff of Ayatollah Rouhani Hospital Babol during 2016. The antibiotic resistance pattern was performed by disc diffusion method for 13 antibiotics, including cefoxitin, cephalothin, teicoplanin, vancomycin, daptomycin, oxacillin, amoxicillin, amikacin, linezolid, ciprofloxacin, levofloxacin, erythromycin, rifampin, according to the CLSI 2015. Polymerase chain reaction (PCR) was used to detect mecA and pvl genes. Finally, the different SCCmec types were determined by multiplex- PCR method.<br>
<span style="color:#0000FF;"><strong><em>Results: </em></strong></span>Among the 120 collected specimens, 40(33.3%) S. aureus isolates were approved. 28(70%) of strains were identified as methicillin-resistant Staphylococcus aureus (MRSA) and the frequency of pvl gene was confirmed 2(5%). Based on the multiplex PCR assay, four different SCCmec types were detected as 35.7% type I, 14.2% type III, 7.1% type II and 3.5% type IV. By a disc diffusion method, no resistance pattern was observed to vancomycin, while 100% of strains were resistant to amoxicillin.<br>
<span style="color:#0000FF;"><strong><em>Conclusion: </em></strong></span>Consequently our results illustrated that isolated S. aureus strains among the staff nasal carriers via mentioned molecular characterization may lead to increase the nosocomial persistent infections in hospitalized patients and also health care workers.</span></span></p>
Staphylococcus aureus, nasal carriers, antibiotic resistance, SCCmec, MRSA
311
316
http://caspjim.com/browse.php?a_code=A-10-740-1&slc_lang=en&sid=1
Parisa
Sabbagh
Parisa.sabbagh@gmail.com
100319475328460010291
100319475328460010291
No
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Amirmorteza
Ebrahimzadeh-Namvar
Amirmorteza.namvar@gmail.com
100319475328460010292
100319475328460010292
No
Department of Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Elaheh
Ferdosi-Shahandashti
elaheh.ferdosi@yahoo.com
100319475328460010293
100319475328460010293
No
Department of Biotechnology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Mostafa
Javanian
M.javanian@gmail.com
100319475328460010294
100319475328460010294
No
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Soraya
Khafri
Soraya.khafri@yahoo.com
100319475328460010295
100319475328460010295
No
Department of Statistics and Epidemiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
Mehdi
Rajabnia
Ramazan69@yahoo.com
100319475328460010296
100319475328460010296
Yes
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.