Caspian Journal of Internal Medicine
1
Caspian J Intern Med
Medical Sciences
http://caspjim.com
1
admin
2008-6164
2008-6172
8
10.22088/cjim
14
8888
13
en
jalali
1397
12
1
gregorian
2019
3
1
10
2
online
1
fulltext
en
In vitro and in vivo anti-diabetic and anti-oxidant activities of methanolic leaf extracts of Ocimum canum
pharmacology
pharmacology
Original Article
Original Article
<span style="font-family:times new roman;"><span style="font-size:14px;"><span style="color:#0000FF;"><strong><em>Background:</em></strong> </span>Diabetes is a metabolic disorder with a highly complex, multifaceted and intricate etiologies and thus may require management options that proffers multimodal mechanism of action. This present study evaluated the antidiabetic and antioxidant potential of the methanolic extract/fractions of leaves of <em>Ocimum canum.</em><br>
<span style="color:#0000FF;"><strong><em>Methods: </em></strong></span>The antidiabetic potential was evaluated and using STZ-induced diabetic Wistar rat model (<em>in vivo</em>) and inhibition of α-amylase and α-glucosidase activity (<em>in vitro</em>). Antioxidant activity was assessed <em>in vitro</em> by free radical scavenging and reducing power assays and <em>in vivo</em> via monitoring SOD and CAT activities; GSH and MDA levels.<br>
<span style="color:#0000FF;"><strong><em>Results: </em></strong></span>The total phenolic content (221.0±3.0 mg catechol/g of sample) and tannins (146.0±4.0 mg tannic acid/g of sample) of the crude extract; and flavonoid of the aqueous-methanol fraction (216.0.0±1.0 mg of rutin/g of sample) were found to be significantly higher relative to others. The crude extract and the aqueous-methanol fraction exhibited a significantly (p<0.05) higher percentage reduction in fasting blood glucose and a concomitant increase in serum insulin level relative to the diabetic control group. The highest radical scavenging activity and reducing power were observed in the aqueous-methanol fraction. The aqueous-methanol solvent fraction also significantly reversed the alterations in oxidative stress markers occasioned by the diabetic condition.<br>
<span style="color:#0000FF;"><strong><em>Conclusion: </em></strong></span>In conclusion, the result of the present study has demonstrated evidently that extracts of <em>Ocimum canum</em> leaves ameliorates hyperglycemia and the associated oxidative stress in STZ-induced rats.</span></span>
Keywords: Diabetes mellitus, Oxidative stress, Ocimum canum, insulin
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http://caspjim.com/browse.php?a_code=A-10-1234-1&slc_lang=en&sid=1
Chimaobi J
Ononamadu
ononamaducj0016@gmail.com
100319475328460016895
100319475328460016895
Yes
Department of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, Kano State, Nigeria
Adamu, J.
Alhassan
ajalhassan@yahoo.com
100319475328460016896
100319475328460016896
No
Department of Biochemistry, Bayero University, Kano State, Nigeria
Abdullahi A.
Imam
aa.mam@yahoo.com
100319475328460016897
100319475328460016897
No
Department of Biochemistry, Bayero University, Kano State, Nigeria
Aminu
Ibrahim
ibrahima2794@buk.edu.ng
100319475328460016898
100319475328460016898
No
Department of Biochemistry, Bayero University, Kano State, Nigeria
Godwin, O.
Ihegboro
goihegboro@yahoo.com,
100319475328460016899
100319475328460016899
No
Dept of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, Kano State
Alowonle, T.
Owolarafe
abufatimawamaryam@gmail.com
100319475328460016900
100319475328460016900
No
Department of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, Kano State, Nigeria
Muhammed, S.
Sule
mssule@yahoo.com
100319475328460016901
100319475328460016901
No
Department of Biochemistry, Bayero University, Kano State, Nigeria