Caspian Journal of Internal Medicine
1
Caspian J Intern Med
Medical Sciences
http://caspjim.com
1
admin
2008-6164
2008-6172
8
10.22088/cjim
14
8888
13
en
jalali
1392
11
1
gregorian
2014
2
1
5
2
online
1
fulltext
en
Efficacy of multiplex PCR procedure for Iranian Streptococcus pneumoniae isolates
Infectious Diseases
Infectious Diseases
Short Communication
Short Communication
Background: Identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. The purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex PCR for the diagnosis of streptococcus pneumonia serotypes.
Methods: All isolated S. pneumonia from suspected patients in Tehran and Isfahan Hospitals from June to December of 2012 were evaluated. All specimens and their serotypes were identified through a conventional method and specific antisera. Serotype specific multiplex PCR was applied and ran in seven reactions consisting of 34 S. pneumonia primer pairs plus a primer pair as an internal control for this purpose.
Results: Overall, 14 genotype specific serotypes (including two subtypes for 19 and 23) were detected and had identical results with stereotyping method except for serotype 28 and one of the identified serotype 23. The serotypes 19, 6 and 23 were dominant with the frequency of 51.8%. A cross reactivity was also observed between genotypes 1 and 9A/9V.
Conclusion: Applied multiplex PCR format can be suitable and cost effective tool for identification of S. pneumonia serotypes.
S. pneumoniae, Multiplex PCR, Identification
109
113
http://caspjim.com/browse.php?a_code=A-10-62-178&slc_lang=en&sid=1
Massoud
Hajia
10031947532846001145
10031947532846001145
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Mohamad
Rahbar
10031947532846001146
10031947532846001146
Yes
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Marjan
Rahnami Farzami
10031947532846001147
10031947532846001147
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Alireza
Dolatyar
10031947532846001148
10031947532846001148
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Mohsen
Imani
10031947532846001149
10031947532846001149
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Roghieh
Saburian
10031947532846001150
10031947532846001150
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.
Mohamad
Farzanehkhah
10031947532846001151
10031947532846001151
No
Health Reference Laboratories Research Center, Ministry of Health & Medical Education, Tehran, Iran.