en Hematology Hematology Short Communication Short Communication <p> <font face="Times New Roman" size="3"> </font><font face="Times New Roman"><b><i><span lang="EN-US" style="color: blue font-size: 10pt">Background: </span></i></b><span lang="EN-US" style="font-size: 10pt">Chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. One of the main mechanisms of resistance to chemotherapy is the increased expression of MDR1 gene. The aim of this study was to explore the effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast of new cases of acute lymphoblastic leukemia (ALL) patients in vitro.<b><i><span style="color: blue"><o:p /></span></i></b></span></font><font face="Times New Roman" size="3"> </font></p><p><font face="Times New Roman"><b><i><span lang="EN-US" style="color: blue font-size: 10pt">Methods:</span></i></b><span lang="EN-US" style="font-size: 10pt"> Separation of lymphoblasts of 5 new case ALL patients from peripheral blood was performed by <a href="https://www.google.com/search?biw=1280&bih=647&q=ficoll+hypaque+density+gradient+centrifugation&revid=2015296685&sa=X&ei=_9Y3VMaVEcnoaLzLgegM&ved=0CG8Q1QIoBw"><span style="color: windowtext text-decoration: none text-underline: none"><font size="2">ficoll density gradient centrifugation</font></span></a>. Lymphoblasts were cultured in RPMI 1640 medium. Lymphoblasts were treated with 50µmol/L resveratrol for 48 h. Total RNA was extracted with guanidine isothiocyanate. RNA was converted to cDNA. Real time PCR was used to detect mRNA expression of MDR1.<span class="longtext"><span style="background: white color: black"><o:p /></span></span></span></font><font face="Times New Roman" size="3"> </font></p><p><font face="Times New Roman"><b><i><span lang="EN-US" style="color: blue font-size: 10pt">Results:</span></i></b><span lang="EN-US" style="font-size: 10pt"> The results of gene detection showed that the expression of MDR1 did not change significantly in the patients however, in one patient expression of MDR1 increased upon treatment with resveratrol.<span class="longtext"><span style="background: white color: black"><o:p /></span></span></span></font></p><font face="Times New Roman" size="3"> </font><font face="Times New Roman"><b><i><span lang="EN-US" style="color: blue font-size: 10pt">Conclusion: </span></i></b><span lang="EN-US" style="font-size: 10pt">The results of this study did not support resveratrol as a compound to reverse multidrug resistance in leukemic lymphoblasts.<o:p /></span></font><font face="Times New Roman" size="3"> </font> Resveratrol, Multi drug resistance gene, Leukemic lymphoblast, ALL 113 115 http://caspjim.com/browse.php?a_code=A-10-265-1&slc_lang=en&sid=1 Abbasali Hoseinpoor feyzi porfeizi@gmail.com 10031947532846003024 10031947532846003024 No Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Majid Farshdousti hagh m.farshdousti@gmail.com 10031947532846003025 10031947532846003025 Yes Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Tohid Ebadi tohid2ebadi@gmail.com 10031947532846003026 10031947532846003026 No . Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Karim Shams-Asanjan k.sh.asanjan@gmail.com 10031947532846003027 10031947532846003027 No . Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Aliakbar Movasaghpoor-Akbari movasagpoor@gmail.com 10031947532846003028 10031947532846003028 No Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Mehdi Talebi mt09143633748@yahoo.com 10031947532846003029 10031947532846003029 No Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Behzad Ebadi emadi@yahoo.com 10031947532846003030 10031947532846003030 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran