Caspian Journal of Internal Medicine
Babol University of Medical Sciences
یکشنبه 21 اردیبهشت 1404
[Archive]
دوره 11، شماره 3 - ( 3-1399 )
جلد 11 شماره 3 صفحات 289-283 |
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Dosunmu A, Uche E, Osikomaiya B, Ismail A, Akinbami A, Akanmu A. Red cell membrane protein abnormalities as defined by sds-page among patients with anaemia in a west african region hospital practice. Caspian J Intern Med 2020; 11 (3) :283-289
URL: http://caspjim.com/article-1-1729-fa.html
URL: http://caspjim.com/article-1-1729-fa.html
Red cell membrane protein abnormalities as defined by sds-page among patients with anaemia in a west african region hospital practice. . 1399; 11 (3) :283-289
چکیده: (4205 مشاهده)
Background: Erythrocytes require an ability to deform and withstand shear stress while negotiating microcirculation. These properties are largely due to their excess surface area per volume and the characteristics of the membrane’s protein. Deficiencies of these proteins are associated with chronic hemolysis. Methods: This was a cross-sectional study aimed at determining the prevalence of red cell membrane protein abnormalities as determined by sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE) among patients with anemia attending the outpatient clinics of the hospital.
Results: A total of 823 participants were recruited into the study with a mean age of 34±14 years. There were 410 (49.8%) participants with hematocrit ≥ 36% and 413 with hematocrit ≤ 35.9% of which 192 participants (23.3%) had abnormal red cell indices. Following SDS-PAGE, 21 (10.9%) of the 192 participants had deficient PAGE tracing. Abnormal spectrin band was observed in 17 (81%) of the 21 participants. The hematocrit was significantly lower while the reticulocyte count and red cell distribution width were higher in participants with red cell membrane abnormalities.
Conclusion: One in ten patients with mild anemia and abnormal red cell indices in clinical practice may be having hereditary red cell membrane protein defect. Presence of raised reticulocyte count, family history of mild anemia, increased red cell distribution width and red cell morphology may be used to screen for membrane deficiency.
Results: A total of 823 participants were recruited into the study with a mean age of 34±14 years. There were 410 (49.8%) participants with hematocrit ≥ 36% and 413 with hematocrit ≤ 35.9% of which 192 participants (23.3%) had abnormal red cell indices. Following SDS-PAGE, 21 (10.9%) of the 192 participants had deficient PAGE tracing. Abnormal spectrin band was observed in 17 (81%) of the 21 participants. The hematocrit was significantly lower while the reticulocyte count and red cell distribution width were higher in participants with red cell membrane abnormalities.
Conclusion: One in ten patients with mild anemia and abnormal red cell indices in clinical practice may be having hereditary red cell membrane protein defect. Presence of raised reticulocyte count, family history of mild anemia, increased red cell distribution width and red cell morphology may be used to screen for membrane deficiency.
نوع مطالعه: Original Article |
موضوع مقاله:
Hematology
دریافت: 1397/10/29 | پذیرش: 1398/11/2 | انتشار: 1399/3/1
دریافت: 1397/10/29 | پذیرش: 1398/11/2 | انتشار: 1399/3/1
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