en Imonology Imonology Original Article Original Article <span style="font-family:times new roman;"><span style="color:#0000FF;"><strong><em>Background</em></strong><em>:</em></span> Recent evidence has indicated that beta-secretase 1 (BACE1) is involved in the production of amyloid beta (A&beta;) in patients affected with Alzheimer&rsquo;s disease (AD). Therefore; the purpose of this study was to measure mRNA and plasma levels of BACE1 in AD patients, as an early diagnosis biomarker for such individuals.<br> <span style="color:#0000FF;"><strong><em>Methods</em></strong><em>:</em></span> A total number of thirty AD patients and thirty normal subjects as controls were recriuted in the present study. Plasma levels of BACE1 were then examined via enzyme-linked immunosorbent assay (ELISA) and also mRNA expression of BACE1 in total blood was measured using real-time PCR technique.<br> <span style="color:#0000FF;"><strong><em>Results</em></strong><em>:</em> </span>The findings revealed a significant difference in gene expression of BACE1 in the peripheral blood of AD patients compared with that in controls (p<0.0001). Additionally, elevated plasma levels of BACE1 were found in AD patients compared with those in normal subjects (p<0.01). Statistical analyses also demonstrated no correlation between expression (mRNA and protein) of BACE1 in both AD patients and controls and age or the results of Mini-Mental State Examination (MMSE) scale (p>0.05).<br> <span style="color:#0000FF;"><strong><em>Conclusion</em></strong><em>:</em> </span>Given the importance of early diagnosis of AD patients, it was suggested that the measurement of plasma levels and also mRNA expression of BACE1 might be a valuable blood-based biomarker used in preference to other invasive diagnostic methods such as cerebrospinal fluid (CSF) analysis.</span> BACE1, Alzheimer’s disease, biomarker 276 280 http://caspjim.com/browse.php?a_code=A-10-1243-1&slc_lang=en&sid=1 Alireza Vakilian alirezavakilian7@gmail.com 100319475328460018053 100319475328460018053 No Non Communicable Diseases Research Center, Rafsanjan University of Medical Science, Rafsanjan, Iran Javad Masoumi masoumi.javad.2024@gmail.com 100319475328460018054 100319475328460018054 No Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Saeid Mirzaee hossein_1392@yahoo.com 100319475328460018055 100319475328460018055 No Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Hossein Khorramdelazad khorramdelazad@gmail.com 100319475328460018056 100319475328460018056 Yes Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran