en Microbiology Microbiology Original Article Original Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="color:#0000ff;"><strong><em>Background</em></strong><em>:</em></span> Multidrug resistance (MDR) in <em>Enterobacter spp.</em> has created therapeutic challenges all over the world. The present study was conducted for evaluating the prevalence of class I integron, determining the gene cassettes and antimicrobial resistance profile of <em>Enterobacter spp.</em> isolates from clinical samples in Babol, North of Iran.<br> <span style="color:#0000ff;"><strong><em>Methods</em></strong><em>:</em></span> During a 13-month period, 30 <em>Enterobacter spp.</em> isolates were collected from Ayatollah Rouhani Hospital, Babol, Iran. Various types of antimicrobial agents were used to determine the resistance pattern. Class I integron and associated gene cassettes were detected by PCR assay.<br> <span style="color:#0000ff;"><strong><em>Results</em></strong><em>:</em></span> The resistance rates to AP, CPM, CTX, TM, NI, IMI, AK, CIP and GM antimicrobials were 100%, 93.3%, 33.3%, 33.3%, 30%, 20%, 20%, 20% and 13.3%, respectively. The distribution results of <em>int</em> genes showed that 63.3% of isolates carried the <em>intI</em> genes. Also, the prevalence of <em>aadB</em>, <em>dfrA1</em>, <em>bla</em>_OXA30and <em>bla</em>_PSE1genes were estimated at 36.6%%, 33.3%, 6.6% and 0%, respectively.<br> <span style="color:#0000ff;"><strong><em>Conclusion</em></strong><em>:</em></span> Our results showed that class I integrons have a widespread distribution among the <em>Enterobacter</em> <em>spp.</em> isolates and have clinical relevance to MDR isolates. The results confirmed the necessity for uninterrupted monitoring to prevent distribution of multidrug resistance among <em>Enterobacter spp</em>. strains in Iran</span></span> class I integron, enterobacter, multi drug resistance (MDR). 65 69 http://caspjim.com/browse.php?a_code=A-10-1640-1&slc_lang=en&sid=1 Elaheh Ghasemi amiujk@gmail.com 100319475328460027342 100319475328460027342 No Department of Biotechnology, Islamic Azad University, Damghan, Iran Elaheh Ferdosi-Shahndashti elaheh.ferdosi@yahoo.com 100319475328460027343 100319475328460027343 Yes Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Mehdi Rajabnia ramazan9@yahoo.com 100319475328460027344 100319475328460027344 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Parisa Sabbagh amir9226012@gmail.com 100319475328460027345 100319475328460027345 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Amirhosein Maali maali.amirhosein@gmail.com 100319475328460027346 100319475328460027346 No Student Research Committee, Pasteur Institute of Iran, Tehran, Iran Azadeh Ferdosi-Shahandashti 100319475328460027347 100319475328460027347 No Department of Microbiology, School of Sciences, Islamic Azad University, Ayatollah Amoli Branch, Amol, Iran