en Biochimestry Biochimestry Review Article Review Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:14pt"><b><i><span style="color:blue">Background:</span></i></b> This study aimed&nbsp;to investigate the efficacy of Jumpstart Nutrition&reg; dietary supplement (JNDS) for enhancing the skeletal muscle metabolism and function of older adults with knee osteoarthritis (KOA) by evaluating the biomarkers of aberrant levels of serum tumor necrosis factor-alpha (TNF-&alpha;), interleukin-10 (IL-10), C-reactive protein (CRP), creatine kinase-muscle (CK-MM), and aldolase-A (Aldo-A). </span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Methods</span></i></b><i><span style="color:blue">:</span></i> This twelve-week registry included 54 patients treated with JNDS mainly comprised of calcium, phosphorus, vitamin-K₂, coenzyme-Q₁₀, boswellic acid, and curcumin mixed with soy and whey protein (experimental group) and 51 patients treated with symptomatic slow-acting drugs for osteoarthritis (SYSADOA) (control group) for KOA confirmed with radiological images. At week 0 and week 12 for both the groups evaluated, the non-fasting serum levels of TNF-&alpha;, IL-10, CRP, CK-MM, and Aldo<span style="background:lime"><span style="background-color:#ffffff;">-A by using appropriate kits</span></span><span style="background-color:#ffffff;">.</span></span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Results</span></i></b><i><span style="color:blue">:</span></i> At week-twelve, the respective values of area under the ROC curves of the studied biomarkers for pooled experimental cohorts were 0.928, 0.907, 0.908, 0.927, and 0.988 having the significance of accuracy (R-square):66.28%, 47.25%, 70.39%, 65.13%, and 68.00%, indicating a satisfactory treatment policy, their mean&plusmn; SD, and risk ratio, all exhibited highly significant differences (p<0.0001) and KOA-gradation was upgraded between&ge;2 and &ge;3 from&ge;4 as per the Kellgren-Lawrence&nbsp; scale compared to the control. Fewer patients had to use emergency medications (p<0.05).</span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Conclusions:</span></i></b> Results suggest that JNDS may be effectively used to strengthen the skeletal muscle metabolism&nbsp;and function of elderly patients with KOA confirmed with the stabilization of studied biomarkers as an alternative to the treatment of SYSAD correlated with ROC curves and the Kellgren-Lawrence scale.<b><i><span style="color:#0033cc"></span></i></b></span></span></span><br> &nbsp; knee osteoarthritis, Jumpstart Nutrition®, dietary supplement, biomarkers, symptomatic slow-acting drugs, skeletal muscle metabolism 590 606 http://caspjim.com/browse.php?a_code=A-10-1136-5&slc_lang=en&sid=1 Apurba Ganguly apurbaganguly15@gmail.com 100319475328460045298 100319475328460045298 Yes Department of Research and Development, OPTM Research Institute, Kolkata, India Banerjee Sudip 100319475328460045299 100319475328460045299 No