en Dentistry Dentistry Original Article Original Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:14pt"><b><i><span style="color:blue">Background</span></i></b><i><span style="color:blue">:</span></i> <span style="background:white">Cone beam computed tomography (CBCT) is considered a common examination for dentistry problems. Cellular biology can be affected by exposure to ionizing radiations procedures. In this study, we aimed to assess the genotoxicity and cytotoxicity effects of CBCT dental examinations at two different fields of view (FOVs) in exfoliated buccal epithelial cells.</span><span style="font-size:9.0pt"></span></span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Methods:</span></i></b> <span style="background:white">Sixty healthy adults participated in the current study. They were divided into two identical groups; CBCT with FOV of 6*6 cm2 and 8*11 cm2. Exfoliated oral mucosa cells were prepared immediately before and after 10-12 days of CBCT exposure. The cytological smears were stained with the Papanicolaou technique. The amounts of micronuclei and other cytotoxicity cellular changes (Pyknosis, Karyolysis, and Karyorrhexis) were evaluated. The variables of the parameters before and after CBCT examination in the two investigated FOVs were performed using Wilcoxon test and paired-samples t-test in SPSS software.</span><span style="font-size:10.0pt"></span></span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Results:</span></i></b> <span style="background:white">The micronuclei and other cytotoxic changes parameters before and after CBCT exposure for both FOVs (6*6 and 8*11 cm2) increased significantly (p<0.001). Furthermore, a significant difference (p<0.05) was observed between the investigated parameters at the two FOVs. Notably, the FOV of 8*11 cm2 had more side effects than that of 6*6 cm2. There were no statistically significant among males and females for both FOVs.</span><b><i><span style="color:blue"></span></i></b></span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Conclusion</span></i></b><i><span style="color:blue">:</span></i> <span style="background:white">CBCT examinations of dental disorders would increase the risks of inducing genetic damage. The cytotoxicity and chromosomal damage were considered in males and females in both investigated FOVs (6*6 and 8*11 cm2). In this regard, the use of CBCT must be following the ALARA principle.</span><b><i><span style="color:#0033cc"></span></i></b></span></span></span> Cone beam computed tomography (CBCT), Oral mucosa epithelium, Genotoxicity, Cytotoxicity, Field of view (FOV) 121 127 http://caspjim.com/browse.php?a_code=A-10-2618-1&slc_lang=en&sid=1 Roghaieh Faeli Ghadikolaei saharfaeli@gmail.com 100319475328460040867 100319475328460040867 No Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran Hakimeh Ghorbani h.ghorbani@mubabol.ac.ir 100319475328460040868 100319475328460040868 No Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Maryam Seyedmajidi Ms_majidi79@yahoo.com 100319475328460040869 100319475328460040869 No Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran Kourosh Ebrahimnejad Gorji K.E.Gorji@gmail.com 100319475328460040870 100319475328460040870 No Department of Medical Physics Radiobiology and Radiation Protection, School of Medicine, Babol University of Medical Sciences, Babol, Iran Ehsan Moudi Ehsan.moudi@gmail.com 100319475328460040871 100319475328460040871 Yes Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Seyedali Seyedmajidi 100319475328460040872 100319475328460040872 No Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran