en Gastroentrology Gastroentrology Original Article Original Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:14pt"><b><i><span style="color:blue">Background</span></i></b><i><span style="color:blue">:</span></i> <span lang="EN">Evidence suggested that olive oil may alleviate constipation symptoms</span>. This study compared the effectiveness of two types of extra virgin olive oil and refined olive oil on the treatment of patients experiencing constipation.</span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Methods:</span></i></b> This double-blind clinical trial included 140 participants aged 18 years or older who were diagnosed with chronic constipation by a gastroenterologist using the Rome III questionnaire. <span lang="EN">Block randomization was utilized </span>approach to divide<span lang="EN"> patients into two groups of 70 individuals (Extra virgin olive oil as trial and refined olive oil as control) that </span>consumed two tablespoons per day of olive oil for four weeks. Follow-up assessments were conducted using the Rome III and Bristol Stool Form Scale (BSFS) questionnaires. </span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Results:</span></i></b> According to analysis of covariance (ANCOVA), <span lang="EN">after four weeks of the intervention, the mean score of Rome III decreased significantly from 11.37&plusmn;2.25 to 7.74&plusmn;2.53; and from 11.27&plusmn;2.73 to 3.47&plusmn;1.73 among refined olive oil and extra virgin olive oil consumers, </span>respectively<span lang="EN">, (-4.22 (</span>95% CI: <span lang="EN">3.63-4.82)</span>, (F (_1,137) =197.67, p<0.001, &eta;2P=0.591)<span lang="EN">. </span>In week 2,3, and 4, the mean score of BSFS were significantly higher among consumers of extra virgin olive oil than to the consumers of refined olive oil 0.53 (95% CI: 0.38- 0.68), p<0.001, <span lang="EN">0.78 (</span>95% CI: <span lang="EN">0.60- 0.97), </span>p<0.001 and 1.14<span lang="EN"> (</span>95% CI: <span lang="EN">0.94-1.35),</span> p<0.001, respectively.</span><br> <span style="line-height:14pt"><b><i><span style="color:blue">Conclusion</span></i></b><i><span style="color:blue">:</span></i> <span lang="EN">Extra virgin olive oil was more effective compared refined olive oil at alleviating constipation. </span>These outcomes highlight the importance of <span lang="EN">extra virgin </span>olive oil as a possible treatment for constipation.<span lang="EN" style="font-size:10.0pt"></span></span></span></span><br> &nbsp; Constipation, Extra virgin olive oil, Rome III, Bristol Stool Form Scale 674 685 http://caspjim.com/browse.php?a_code=A-10-2942-8&slc_lang=en&sid=1 Farahnaz Joukar farajov@gmail.com 100319475328460054604 100319475328460054604 No Guilan University of Medical Sciences, Rasht, Iran Seyedeh Narjes Mozaffari Chenijani milad_ebrahimnejad@yahoo.com 100319475328460054605 100319475328460054605 No Guilan University of Medical Sciences, Rasht, Iran Saman Maroufizadeh Saman.maroufizadeh@gmail.com 100319475328460054606 100319475328460054606 No Guilan University of Medical Sciences, Rasht, Iran Tahereh Zeinali tarane.zeinali@gmail.com 100319475328460054607 100319475328460054607 No Guilan University of Medical Sciences, Rasht, Iran Niloofar Faraji niloofarfaraji.sci@gmail.com 100319475328460054608 100319475328460054608 No Guilan University of Medical Sciences, Rasht, Iran Narges Eslami negareslami070@gmail.com 100319475328460054609 100319475328460054609 No Guilan University of Medical Sciences, Rasht, Iran Fariborz Mansour-Ghanaei fmansourghanaei@gmail.com 100319475328460054610 100319475328460054610 Yes Guilan University of Medical Sciences, Rasht, Iran