Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Helicobacter pylori infection and osteoporosis in elderly patients
48
50
EN
Behzad
Heidari
Babol University of Medical Sciences
changes (BMD) and osteoporosis .Helico bacter pylori which is a known cause of gastritis and peptic ulcer Osteopprosis is one major cause of bone fracture and subsequent morbidity and mortality in the elderly population. Several clinical and demographic parameters including aging, menopause, parity , inflammatory disease, hormonal, gastrointestinal, renal and metabolic disorders like vitamin D deficiency, obesity, weigh loss may affect bone mass and result in bone mineral density disease is associated with systemic inflammation. It has been also linked to many other disorders like autoimmune thyroid diseases, diabetes mellitus, dyslipidemia, obesity, osteoporosis and primary hyperparathyroidism.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Metabolic syndrome and the associated risk factors in Iranian adults: A systematic review
51
61
EN
Karimollah
Hajian-Tilaki
Babol University of Medical Sciences
Background: Metabolic syndrome (MetS) is a complex
clustering cardiovascular risk factors such as abdominal obesity, hypertension,
diabetes and dyslipidemia. It has been a growing health problem in Iranian
adults in recent decade. The objective of this article was to review the
prevalence of MetS and the corresponding risk factors among Iranian adults.Methods: We conducted a systematic review to
extract the published articles regarding metabolic syndrome and its risk
factors among Iranian adults aged >19 years by searching in PubMed, Google
Scholar, SID, Magiran and Iranmedex databases. The forty-three published
articles were selected regarding MetS among Iranian adults in this review
during 2005-2014.Results: From the 43 studies, the rate of MetS
varied from 10% to 60% depending on sex, age and region. The highest rate
reported among postmenopausal women in Shiraz was over 60%. There was almost a
consistent finding that the rate of MetS was higher among women compared with
men across national level except in one study. A very sharp difference (43.3%
vs. 17.1%) was observed in western Iran (Kordestan province) between sexes.
MetS was significantly more prevalent among older adults, postmenopausal women,
less-educated people, those living in urban areas and those with low physical
activity and unhealthy eating habits across national level consistently.Conclusion: An emerging high rate of MetS across
national level highlights the lifestyle modification as preventive measures in
Iranian population by focusing primarily on high risk profiles such as low
socioeconomic background, low level of education, older age and postmenopausal
women.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Relationship between Helicobacter pylori infection (HP) and bone mineral density (BMD) in elderly people
62
66
EN
Mahdiye
Fotouk-kiai
Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran.
Seyed Reza
Hoseini
Social Determinates of Health Research Center, Health Research Institute, Babol University of Medical Sciences Babol, Iran.
Neda
Meftah
. Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran.
Reza
Ghadimi
Social Determinates of Health Research Center, Health Research Institute, Babol University of Medical Sciences Babol, Iran.
Ali
Bijani
Social Determinates of Health Research Center, Health Research Institute, Babol University of Medical Sciences Babol, Iran.
Hajighorban
Noreddini
Department of Radiology, Babol University of Medical Sciences, Babol, Iran.
Hamidreza
Nematollahi
Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran.
Javad
shokri-shirvani
Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran
Background: Low bone mass is a frequent complication of
chronic inflammatory disease. The pathogenesis of osteoporosis in chronic inflammatory
disease may be secondary to releases of cytokines such as TNF-a and IL6.
Chronic gastritis due to helicobacter pylori (HP) infection may lead to
decreased bone mineral density (BMD) and predispose patients to osteoporosis.
The objective of this study was to determine the BMD status in HP positive patients
with gastritis versus HP negative cases. Methods: In this cross-sectional study, we enrolled
967 participants aged 60 years old and more from Amirkola Health Study Ageing
Project. Seven-hundred and fifty eight HP positive and 209 HP negative patients
were analyzed. BMD was measured by dual-energy x-ray absorptiometry (DXA)
method in the spine and femoral neck in all participants. Results: The mean age in HP+ and HP- negative
patients was 68.3±6.8 and 69.3±7.4 years, respectively. BMD g/cm2 in the spine
and femoral neck did not differ between the two groups (P=0.19 and 0.22
respectively). The prevalence of osteoporosis did not also differ across the
two groups as well. There was no relationship between the level of antibodies
against HP and BMD.Conclusion: According to the findings of this study, H.
pylori infection is not associated with BMD changes in the elderly population.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Women’s satisfaction in early versus delayed postcaesarean feeding: A one-blind randomized controlled trial study
67
71
EN
Shahnaz
Barat
Obstetrics and Gynecology Department, Babol University of Medical Sciences, Babol, Iran.
Sedigheh
Esmaeilzadeh
Fatemezahra Infertility and Reproductive Health Research Center, Babol University of Medical Sciences, Babol, Iran.
Masoumeh
Golsorkhtabaramiri
Obstetrics and Gynecology Department, Babol University of Medical Sciences, Babol, Iran.
Soraya
Khafri
Social Medicine Department, Babol University of Medical Sciences, Babol, Iran
Maryam
Moradi Recabdarkolaee
Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Background: The early
postoperative feeding after caesarean
section (C-
section) has remained controversial. This study was designed to evaluate the
safety and efficacy of early versus delayed postcaesarean section oral feeding
regarding gastrointestinal complications and patients postoperative satisfaction
after C-
section. Methods: This clinical trial study was conducted on
200 pregnant women undergoing planned C-section under
spinal anesthesia (Registration Number: IRCT: 138712211760N1). Women were randomly divided in
two groups group A (early feeding group) comprised of 101 patients who were
encouraged to take oral fluid. If they tolerated, they continue semi-solid and
solid foods starting 2 h after caesarean section. Group B (delayed
feeding group) comprised of 99 patients who were given oral fluid 8 h after
surgery. After beginning of feeding the patients’ tolerance,
first flatus, first defecation, beginning of regular diet, the length of
hospital stay and also patient satisfaction level were evaluated in each group by
visual scale analog (VAS). Results: The mean time of the first passage of flatus
was 10.2±1.7 hours for the early oral feeding group versus 10.7±1.6 hours for the
delayed feeding group and the difference was significant (P=0.03). Duration to
first defecation and length of
hospital stay as well as
patient satisfaction level did not differ significantly between the two groups.
Conclusion: The results of this study suggest early postcaesarean
feeding. It is well tolerated and helps return normal feeding habits.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Influence of thyroidectomy on postoperative serum calcium level regarding serum vitamin D status. A prospective study
72
76
EN
Gholamali
Godazandeh
Department of Thoracic Surgery, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran
Zahra
Kashi
Diabetes Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Farnaz
Godazandeh
Department of Radiology, Imam Khomeini Hospital , Mazandaran University of Medical Sciences, Sari, Iran.
Pouya
Tayebi
Department of Thoracic Surgery, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
Ali
Bijani
Social Determinant of Health Research Center, Babol University of Medical Sciences, Babol, Iran.
Background: Hypocalcemia is a
well-recognized complication after total thyroidectomy. Hypovitaminosis D may
have additional effect in the development of hypocalcemia. This study aimed to
determine the effect of total thyroidectomy on postoperative serum calcium in
patients with and without hypovitaminosis D. Methods: This prospective
study was performed on patients who underwent total thyroidectomy from 2011 to
2014 in Imam Khomeini General Hospital of Mazandaran University of Medical
Sciences. Serum calcium and vitamin D values were recorded before and after
surgery. The patients were classified according to serum vitamin D concentrations
as less 10 ng/ml (vitamin D deficiency) or higher (control group). The mean
values of postoperative calcium level for each class of serum vitamin D were
determined and compared. Hypocalcemia was defined as a postoperative calcium
level <8 mg/dl. Results: 125 patients due
to thyroid disease underwent total thyroidectomy. The incidence of symptomatic
and asymptomatic hypocalcemia after surgery was 12% (n=15) and 3.2% (n=4)
respectively. 82 (65.6%) patients had vitamin D deficiency and 43 (34.4%) patients
had sufficient vitamin D level. There was not any significant difference in
calcium level (8.67±0.58 mg/dl vs. 8.70±0.59 mg/dl) between two vitamin D
studied groups after thyroid surgery (p>0.05). Conclusion: The findings of this study
indicated that vitamin D deficiency had no significant effect on
post-thyroidectomy serum calcium level.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Determination of serum lipid profile in patients with diabetic macular edema that referred to Shahid Beheshti and Ayatollah Rouhani Hospitals, Babol during 2011-2012
77
81
EN
Seyed Ahmad
Rasoulinejad
. Babol University of Medical Sciences, Babol, Mazandaran, Iran.
Habib-ollah
Iri
Department of Ophtalmology, Babol University of Medical Sciences, Babol, Iran.
Background: Diabetes is a common metabolic disorder
leading to the development of many complications, among which diabetic
retinopathy and macular edema are the most significant. These complications can
contribute to blindness if not diagnosed or treated properly, and several
studies have been conducted to evaluate the methods for the prevention or
slowing down their progression. Therefore, serum lipids, apparently play an
effective role in the creation and acceleration of macular edema, we therefore
determined the relationship of serum
lipid level in patients with diabetic macular edema in the present study. Methods: 180 participants were selected from
patients with the definite diagnosis of diabetes referred to the eye clinic of
Shahid Beheshti and Ayatollah Rouhani Hospitals of Babol during 2011-2012, the
patients with a history of taking lipid –lowering drugs and hypertension were
excluded from the study. The study data were provided from the medical records
of each patients. SPSS Version 18 was used for analyses. Results: In the present investigation, the mean age
of participants was 53.22±with the age range of 18-77 years. Ninety patients
with diabetic retinopathy and macular edema were compared with ninety patients
with diabetic retinopathy without macular edema (control group) were compared. There
was a significant difference in serum cholesterol and LDL-cholesterol between patients
and groups (p<0.000). Conclusion: The results of this study indicate that
high serum cholesterol and LDL-cholesterol is associated with severity of
diabetic retinopathy particularly with macular edema
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Age distribution types of bladder cancers and their relationship with opium consumption and smoking
82
86
EN
Arsalan
Aliramaji
Department of Urology, Babol University of Medical Sciences, Babol, Iran
Aliakbar
Kasaeean
Department of Urology, Babol University of Medical Sciences, Babol, Iran
Yousefreza
Yousefniapasha
Department of Urology, Babol University of Medical Sciences, Babol, Iran
Hamid
Shafi
Department of Urology, Babol University of Medical Sciences, Babol, Iran
Sekineh
Kamali
Clinical Research Development Center, Babol University of Medical Sciences, Babol, Iran.
Mohsen
Safari
Babol University of Medical Sciences, Babol, Iran
Emad
Moudi
Department of Urology, Babol University of Medical Sciences, Babol, Iran
Background: Recognition of the predisposing factors of
bladder cancer is very important and provides possible prevention measures. The
aim of this study was to investigate the types, distribution of bladder tumors
and their relationship with opium consumption and smoking in patients who
referred to Shahid Beheshti Hospital, Babol, Iran. Methods: In this case-control study, all patients
diagnosed with bladder cancer who underwent surgery during 2001-2012 were
enrolled. The subjects of the control group were selected among the patients
who underwent ERCP (endoscopic retrograde cholangiopancreatography) for
gallstone and had no tumors and genitourinary problems. Data regarding
demographic, pathology reports and tumor type, smoking status, history of opium
consumption and its duration were collected. Patients and controls were
compared using t-test and chi-square test. SPSS software Version 20 was used for
analysis. Results: In this study, 175 patients with an
average age of 63.30±15.29
years and 175 age- matched controls were studied. A significant association was
observed between smoking and opium consumption with bladder cancer (P=0.001 for
both). Conclusion: The results of this study showed that opium
consumption and smoking are associated with bladder cancer
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Comparison of the analgesic effect of intravenous paracetamol/midazolam and fentanyl in preparation
87
92
EN
Abbasali
Ahmadi
Parviz
Amri
Javad
Shokri-Shirvani
Karimollah
Hajian
Background: Although some patients can tolerate
colonoscopy procedure using fentanyl/ midazolam without any sedation and
analgesic requirements but some patients may require additional sedation with
benzodiazepines. We performed the present study to compare the effect of
paracetamol/midazolam with fentanyl/ midazolam. Methods: In a clinical trial, 96 patients aged 18
to 75 years old, who were candidate for elective colonoscopy assigned
consecutively into two groups as paracetamol/midazolam and fentanyl/midazolam.
The first group received 1 gr paracetamol 45 minutes before colonoscopy and 0.5
mg/kg midazolam 5 minutes before colonoscopy whereas the second group received 04-
0.5-1 mcg/kg fentanyl 3 minutes before colonoscopy and similar dose of
midazolam. The two groups were compared in regard to patient intensity,
discomfort, a colonoscopist and, patient satisfaction and rescue dose
of propofol during colonoscopy and vital signs. Results: There was no significant difference
between the two groups for patient pain score, colonoscopist satisfaction,
patient satisfaction and rescue dose of propofol (P=0.817, 0.978, 0.460, and 0.104, respectively). The incidence
of apnea was greater in fentanyl group (P=0.045). After adjusting for age and
education, there was also no significant difference between the two groups. Conclusion: This study indicates that paracetamol can
be considered as an alternative drug regimen in preparation of colonoscopy.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Vitamin D status and distribution in patients with chronic obstructive pulmonary disease versus healthy controls
93
97
EN
Behzad
Heidari
mobility impairment research center
Yahya
Javadian
mobility impairment research center
Mahmoud
Monadi
Department of Internal Medicine, Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Yahya
Dankoub
Department of Internal Medicine, Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Alireza
Firouzjahi
Department of Laboratory Medicine, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
Background: Vitamin D has a potential to modulate
inflammatory response against noxious particles in patients with chronic
obstructive pulmonary disease (COPD). The present study was conducted to
determine the status of serum vitamin D in COPD versus healthy group. Methods: The patients presented to the outpatient
pulmonary clinic of Ayatollah Rouhani Hospital, Babol Iran. Diagnosis of COPD
was confirmed based on airflow limitation defined as FEV1/FVC ratio <70% and
FEV1< 80% of predicted. All eligible patients aged ≥ 40 years old entered
the study. Pulmonary infection, tuberculosis, pleural effusion, congestive
heart failure, pulmonary hypertension and embolism, restrictive airway disease,
conditions leading changes in vitamin D metabolism and absorption were
excluded. Serum 25-hydroxyvitamin D (25-OHD) was determined by
electrocheminluminescence method and levels 50 years old. All patients
were males and age and sex-matched controls were selected among healthy
subjects accompanied COPD patients. Results: Ninety patients and 100 controls with
respective mean (±SD) age of 64.8±11.7 and 62.6±11.7 years old (P=0.19) were
studied. Compared with control, proportions of serum 25-OHD deficiency and
insufficiency in patients >50 years were higher and deficiency was lower
(61.5% vs 87.5%, P=0.11). Conclusion: These findings indicate that a significant
proportion of young COPD patients have insufficient serum 25-OHD. Regarding a positive
relationship between 25-OHD and FEV1 in COPD, these findings highlight serum
25-OHD assessment in COPD for recognizing high risk patients.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Influence of core stability exercise on lumbar vertebral instability in patients presented with chronic low back pain: A randomized clinical trial
98
102
EN
Yahya
Javadian
Department of Physiotherapy, Babol University of Medical Sciences, Babol, Iran
Mohammad
Akbari
Department of Physiotherapy, Iran University of Medical Sciences, Tehran, Iran
Ghadamali
Talebi
Department of Physiotherapy, Babol University of Medical Sciences, Babol, Iran.
Mohammad
Taghipour-Darzi
Department of Physiotherapy, Babol University of Medical Sciences, Babol, Iran.
Naser
Janmohammadi
Department of Orthopedy, Babol University of Medical Sciences, Babol, Iran.
Background: Excessive lumbar vertebrae translation and
rotation in sagittal plane has been attributed as an associated factor of
lumbar segmental instability (LSI) and low back pain (LBP). Reduction of these
abnormalities improves back pain. The aim of this study was to investigate the
effect of core stability exercise on the translation and rotation of lumbar
vertebrae in sagittal plane in patients with nonspecific chronic LBP (NSCLBP). Methods: In this randomized clinical trial, 30
patients with NSCLBP due to LSI were included. The
participants were randomly divided into two groups of treatment and control.
The treatment group received general exercises plus core stability exercise for
8 weeks whereas the control group received only general exercises. The magnitude of translation (mm) and rotation
(deg) of lumbar vertebrae in the sagittal plane was determined by radiography
in flexion and extension at baseline and after intervention. The primary
outcome measures were to determine the mean changes from baseline in translation and rotation of the lumbar vertebrae in the
sagittal plane after 8 weeks of intervention in each group. The
secondary outcome was to compare the two groups in regard to translation and
rotation of the lumbar vertebrae at the end of the study period. Data were
analyzed using paired t-test and independent t-test. Results: Thirty patients aged 18-40 years old with
clinical diagnosis of NSCLBP entered the study. Compared with baseline values,
mean value of translation and rotation of the lumbar vertebra reduced
significantly in both groups (P<0.05), except L3 translation in the control
group. At the endpoint, mean translation value of L4 (P=0.04) and L5 (P=0.001)
and rotation of the L5 (P=0.01) in the treatment group was significantly lower
than the control group.Conclusion: These findings indicate that in patients
presented with NSCLBP due to lumbar segmental instability, core stability exercises plus general exercises are more
efficient than general exercises alone in the improvement of excessive lumbar vertebrae translation and rotation.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Immunohistochemical expression of CD10 in cutaneous basal and squamous cell carcinomas
103
107
EN
shahryar
shafaei
Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
mahnaz
sharifian
Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
karimolah
hajian
Department of Biostatistics and Epidemiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Background: Non-melanoma skin cancer is
the most common malignant tumor in humans. The role of ultraviolet radiation is
well-known in the pathogenesis of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). CD10 is a
zinc-dependent metallopeptidase known as common acute lymphoblastic leukemia
antigen (CALLA). Although CD10 expression has been investigated in some
cutaneous tumors, to our knowledge, data regarding its expression in cutaneous
epithelial neoplasms are very limited. In this study, we aimed to determine the
immunohistochemical expression of CD10 in BCC and SCC and to find whether it
could distinguish between these two skin malignancies. Methods: Twenty SCC and 42 BCC cases were retrieved
randomly from Ayatollah Rouhani Hospital pathology archive and CD10 expression
was determined in tumoral and stromal cells of each case based on
immunohistochemical method. Positive CD10 staining was identified as brown
cytoplasmic, with or without cell membrane staining. Results: In all the 20 SCC cases, tumor cells
failed to stain with CD10 in contrast to the stromal cells that showed CD10
expression in 18 cases (90%). In BCC cases, the expression of CD10 was noted in
tumor cells in 25 cases (59.5%) and in stromal cells of 32 cases (76.2%). There
was no relation between CD10 expression in aggressive and non- aggressive BCC. Conclusion: Our findings suggest that CD10 is a useful
immunohistochemical marker to differentiate between BCC and SCC. At least, if
tumor cells were CD10 positive, this would favor BCC over SCC. Due to small
number of aggressive BCC in contrast to non- aggressive types, more studies need
to be done to prove or rule out this finding.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Clinical presentation of coronary arteriovenous fistula according to age and anatomic orientation
108
112
EN
Hassan
zamani
Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran
Mahmoud
Meraji
Pediatric Cardiology Department of Shahid Rajaie Heart Center, Iran University of Medical Sciences, Tehran, Iran
Mohammad Yousef
Arabi Moghadam
Pediatric Cardiology Department of Shahid Rajaie Heart Center, Iran University of Medical Sciences, Tehran, Iran
Behzad
Alizadeh
Mashhad University of Medical Sciences, Mashhad. Iran.
Kazem
Babazadeh
Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran
Farzad
Mokhtari-Esbuie
Mazandaran University of Medical Science, Sari , Iran
Background: Coronary arteriovenous fistulas (CAVFs) are
direct connections from one or more coronary arteries to cardiac chambers or a
large vessel. They are mostly of congenital origin. The aim of this study was
to describe clinical presentation and also delineate the course and management
of CAVF. Methods: Clinical data, chest x-rays,
echocardiographic and angiographic evaluation of 40 patients with congenital
CAVF during 1990 to 2008 were reviewed retrospectively. Results: Seventeen patients were ≤ 20 years old
(42.5%) were mostly asymptomatic, and twenty tree cases were older than 20
years old (57.5%), mostly symptomatic (P<0.05). Twenty one (52.5%) patients
had pure CAVF and nineteen (47.5%) patients with associated intarcardiac
congenital heart disease (15%) or acquired valvular and coronary arteries
diseases (32.5%). CAVFs mostly originated from left anterior descending artery
(LAD) (42.5%) and mostly drained into the main pulmonary artery (MPA) (35 %).
Twenty-four patients underwent CAVF surgical ligation. From twenty-one patients
with pure CAVF, eight (38%) patients were complicated by congestive heart
failure and aneurism formation of fistula.Conclusion: Unlike some previous reports, in our study,
the most prevalent origin site for CAVFs was the left anterior descending
(LAD). Most patients with CAVFs especially those who went first diagnosed
before 20 years old were asymptomatic. On the other hand, as the continuous
murmur is not always detected in children or infants, consequently, cases of
spontaneous closure may remain undetected. All symptomatic and asymptomatic
patients with moderate to severe shunting should be operated on and minimal morbidity
and good surgical results could be expected.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients
113
115
EN
Abbasali
Hoseinpoor feyzi
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Majid
Farshdousti hagh
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Tohid
Ebadi
. Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Karim
Shams-Asanjan
. Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Aliakbar
Movasaghpoor-Akbari
Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Mehdi
Talebi
Department of Hematology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Behzad
Ebadi
Department of Microbiology, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran
Background: Chemotherapy plays a very important role in
the treatment of leukemia but the resistance properties of the lymphoblasts
limit the effect of chemotherapy. One of the main mechanisms of resistance to
chemotherapy is the increased expression of MDR1 gene. The aim of this study
was to explore the effect of resveratrol on the expression of MDR1 gene in
leukemic lymphoblast of new cases of acute lymphoblastic leukemia (ALL)
patients in vitro. Methods: Separation of lymphoblasts of 5 new case
ALL patients from peripheral blood was performed by ficoll
density gradient centrifugation. Lymphoblasts were cultured in RPMI
1640 medium. Lymphoblasts were treated with 50µmol/L resveratrol for 48 h.
Total RNA was extracted with guanidine isothiocyanate. RNA was converted to
cDNA. Real time PCR was used to detect mRNA expression of MDR1. Results: The results of gene detection showed that
the expression of MDR1 did not change significantly in the patients however, in
one patient expression of MDR1 increased upon treatment with resveratrol. Conclusion: The results of this study did not support
resveratrol as a compound to reverse multidrug resistance in leukemic
lymphoblasts.
Babol University of Medical Sciences
Caspian Journal of Internal Medicine
2008-6164
6
2
2015
4
1
Prevalence of influenza A/H3N2 virus in northern Iran from 2011 to 2013
116
119
EN
Mohammadreza
Haghshenas
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Elham
Jafarian
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
Farhang
Babamahmoodi
Antimicrobial Resistance Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Ahmad
Tabrizi
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Sharbano
Nandoost
. Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Reza
Alizadeh-Navaei
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Background: Influenza A virus is the most virulent
human pathogen and causes the most serious problem. Having epidemiological
knowledge about this disease is important. The aim of this study was to
determine the prevalence of influenza A/H3N2 virus infection in northern Iran
from 2011 to 2013 using the real-time polymerase chain reaction (RT-PCR). Methods: In this cross-sectional study 57 samples
were collected from patients with influenza-like illness (T≥ 38 °C and cough or
sore throat. Influenza-RNA was extracted from the samples using PureLinkTM Viral
RNA/DNA Kit. RT-PCR was one using SuperScript III Platinum, Quantitive Real
Time PCR system from invitrogen with
a specific type of primers and probs.
All samples were examined in the Influenza laboratory of Mazandaran University
of Medical Sciences. Results: The mean age of patients was 38.2±14.5
year, 278 (48.69%) were males and 293 (51.31%) females. A total number of 201
patients (35.2%) were diagnosed as influenza A1 H3 N2 infection. Conclusion: The results show that the prevalence of A/H3N2
in the North of Iran is considerable and needs more attention for preventive
measures