Caspian Journal of Internal Medicine
Babol University of Medical Sciences
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URL: http://caspjim.com/article-1-505-en.html
, Elham Jafarian , Farhang Babamahmoodi , Ahmad Tabrizi , Sharbano Nandoost , Reza Alizadeh-Navaei
Background: Influenza A virus is the most virulent
human pathogen and causes the most serious problem. Having epidemiological
knowledge about this disease is important. The aim of this study was to
determine the prevalence of influenza A/H3N2 virus infection in northern Iran
from 2011 to 2013 using the real-time polymerase chain reaction (RT-PCR).
Methods: In this cross-sectional study 57 samples
were collected from patients with influenza-like illness (T≥ 38 °C and cough or
sore throat. Influenza-RNA was extracted from the samples using PureLinkTM Viral
RNA/DNA Kit. RT-PCR was one using SuperScript III Platinum, Quantitive Real
Time PCR system from invitrogen with
a specific type of primers and probs.
All samples were examined in the Influenza laboratory of Mazandaran University
of Medical Sciences.
Results: The mean age of patients was 38.2±14.5
year, 278 (48.69%) were males and 293 (51.31%) females. A total number of 201
patients (35.2%) were diagnosed as influenza A1 H3 N2 infection.
Received: 2015/03/17 | Accepted: 2015/03/17 | Published: 2015/03/17
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