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:: Volume 11, Issue 1 (1-2020) ::
Caspian J Intern Med 2020, 11(1): 75-82 Back to browse issues page
Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma
Nazanin Bagherlou , Safar Farajnia * , Saber Zahri , Ali Dastranj Tabrizi , Atefeh Nazari
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , farajnias@tbzmed.ac.ir
Abstract:   (184 Views)
Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. K-ras codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k-ras gene mutations and CA125 tumor marker in patients with ovarian carcinoma in Tabriz city.
Methods: Genomic DNA was extracted from 67 tissues pertained to women with ovarian carcinoma. Mutations in codon 12 and 13 of k-ras gene were analyzed by Nested PCR and RFLP methods. Titer of CA125 tumor marker was determined by ELISA.
Results: Among the 67 patients, 7 patients (10.4%) had mutation in k-ras codon 12 while none of them had mutation in k-ras codon 13. Based on the results, the frequency of various genotypes were 89.55%, 10.44%, and 0%, for GG, GA, and AA alleles, respectively. The p-value for stage I and Grade I tumors were 0.022 and 0.04, respectively, indicating a statistically significant correlation between codon 12 mutation and stage I and Grade I tumors. Furthermore, we found significant correlations among CA125 tumor marker titers and histological grade (p<0.000) and stage (p<0.000). The mean serum CA125 tumor marker levels in malignant carcinomas were 499.84 U/ml.
Conclusion: The results in this study indicated high prevalence of k-ras codon 12 mutations and high titer of CA125 tumor marker in patients with ovarian carcinoma in the study region.

 
Keywords: CA125, codon 12, k-ras, RFLP, ovarian carcinoma.
Full-Text [PDF 454 kb]   (34 Downloads)    
Type of Study: Original Article | Subject: Genetics
Received: 2019/01/13 | Accepted: 2019/09/25 | Published: 2019/12/17
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Bagherlou N, Farajnia S, Zahri S, Dastranj Tabrizi A, Nazari A. Prevalence of K-RAS mutations and CA125 tumor marker in patients with ovarian carcinoma. Caspian J Intern Med. 2020; 11 (1) :75-82
URL: http://caspjim.com/article-1-1722-en.html


Volume 11, Issue 1 (1-2020) Back to browse issues page
Caspian Journal of Internal Medicine
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