Caspian Journal of Internal Medicine
Babol University of Medical Sciences
Sun, Oct 19, 2025
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Volume 16, Issue 4 (Autumn 2025)
Caspian J Intern Med 2025, 16(4): 725-730 |
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Rezaei Ghamsari R, Shayestehpour M, Sobhani Nasab A, Nazari-Alam A. Zinc oxide nanoparticles as a new approach to eradicate the biofilm of Pseudomonas aeruginosa isolated from clinical samples. Caspian J Intern Med 2025; 16 (4) :725-730
URL: http://caspjim.com/article-1-4571-en.html
URL: http://caspjim.com/article-1-4571-en.html
Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran , shayesteh2009@yahoo.co.uk
Abstract: (57 Views)
Background: Zinc oxide (ZnO) nanoparticles are particularly notable in the biomedical field, especially for antimicrobial applications, due to their beneficial properties. This study aimed to investigate the antimicrobial activity of ZnO nanoparticles against Pseudomonas aeruginosa (P. aeruginosa) isolated from clinical samples.
Methods: A microdilution test was conducted to assess the effectiveness of the synthesized against P. aeruginosa. Additionally, the impact of the zinc oxide nanocomposite on the expression of biofilm-related genes, including algD, lecA, and lecB, was measured using the Real-Time PCR method.
Results: The minimum inhibitory concentration (MIC) of the ZnO nanocomposite against P. aeruginosa was found to be 625 µg/ml. At half the MIC concentration, ZnO nanoparticles demonstrated a 54% inhibition of biofilm formation. The expression levels of the algD, lecA, and lecB genes were reduced by 3.3 (p < 0.001), 1.8 (p < 0.05), and 3.1 folds (p < 0.05), respectively.
Conclusion: Overall, ZnO nanoparticles, by reducing the expression levels of the algD, LecA, and LecB genes in P. aeruginosa, can be utilized as a novel therapeutic approach to improve and treat infection-related biofilms.
Methods: A microdilution test was conducted to assess the effectiveness of the synthesized against P. aeruginosa. Additionally, the impact of the zinc oxide nanocomposite on the expression of biofilm-related genes, including algD, lecA, and lecB, was measured using the Real-Time PCR method.
Results: The minimum inhibitory concentration (MIC) of the ZnO nanocomposite against P. aeruginosa was found to be 625 µg/ml. At half the MIC concentration, ZnO nanoparticles demonstrated a 54% inhibition of biofilm formation. The expression levels of the algD, lecA, and lecB genes were reduced by 3.3 (p < 0.001), 1.8 (p < 0.05), and 3.1 folds (p < 0.05), respectively.
Conclusion: Overall, ZnO nanoparticles, by reducing the expression levels of the algD, LecA, and LecB genes in P. aeruginosa, can be utilized as a novel therapeutic approach to improve and treat infection-related biofilms.
Policy Brief: Original Article |
Subject:
Microbiology
Received: 2024/11/1 | Accepted: 2024/12/31 | Published: 2025/09/18
Received: 2024/11/1 | Accepted: 2024/12/31 | Published: 2025/09/18
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