Caspian Journal of Internal Medicine
Babol University of Medical Sciences
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URL: http://caspjim.com/article-1-427-en.html
, Majid Farshdousti hagh * , Tohid Ebadi , Karim Shams-Asanjan , Aliakbar Movasaghpoor-Akbari , Mehdi Talebi , Behzad Ebadi
Background: Chemotherapy plays a very important role in
the treatment of leukemia but the resistance properties of the lymphoblasts
limit the effect of chemotherapy. One of the main mechanisms of resistance to
chemotherapy is the increased expression of MDR1 gene. The aim of this study
was to explore the effect of resveratrol on the expression of MDR1 gene in
leukemic lymphoblast of new cases of acute lymphoblastic leukemia (ALL)
patients in vitro.
Methods: Separation of lymphoblasts of 5 new case
ALL patients from peripheral blood was performed by ficoll
density gradient centrifugation. Lymphoblasts were cultured in RPMI
1640 medium. Lymphoblasts were treated with 50µmol/L resveratrol for 48 h.
Total RNA was extracted with guanidine isothiocyanate. RNA was converted to
cDNA. Real time PCR was used to detect mRNA expression of MDR1.
Results: The results of gene detection showed that
the expression of MDR1 did not change significantly in the patients however, in
one patient expression of MDR1 increased upon treatment with resveratrol.
Received: 2014/10/30 | Accepted: 2015/01/10 | Published: 2015/03/17
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