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:: Volume 6, Issue 2 (4-2015) ::
Caspian J Intern Med 2015, 6(2): 113-115 Back to browse issues page
The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients
Abbasali Hoseinpoor feyzi , Majid Farshdousti hagh * , Tohid Ebadi , Karim Shams-Asanjan , Aliakbar Movasaghpoor-Akbari , Mehdi Talebi , Behzad Ebadi
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , m.farshdousti@gmail.com
Abstract:   (7564 Views)

 Background: Chemotherapy plays a very important role in the treatment of leukemia but the resistance properties of the lymphoblasts limit the effect of chemotherapy. One of the main mechanisms of resistance to chemotherapy is the increased expression of MDR1 gene. The aim of this study was to explore the effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast of new cases of acute lymphoblastic leukemia (ALL) patients in vitro. 

Methods: Separation of lymphoblasts of 5 new case ALL patients from peripheral blood was performed by ficoll density gradient centrifugation. Lymphoblasts were cultured in RPMI 1640 medium. Lymphoblasts were treated with 50µmol/L resveratrol for 48 h. Total RNA was extracted with guanidine isothiocyanate. RNA was converted to cDNA. Real time PCR was used to detect mRNA expression of MDR1. 

Results: The results of gene detection showed that the expression of MDR1 did not change significantly in the patients however, in one patient expression of MDR1 increased upon treatment with resveratrol.

 Conclusion: The results of this study did not support resveratrol as a compound to reverse multidrug resistance in leukemic lymphoblasts. 
Keywords: Resveratrol, Multi drug resistance gene, Leukemic lymphoblast, ALL
Full-Text [PDF 391 kb]   (1901 Downloads)    
Type of Study: Short Communication | Subject: Hematology
Received: 2014/10/30 | Accepted: 2015/01/10 | Published: 2015/03/17
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Hoseinpoor feyzi A, Farshdousti hagh M, Ebadi T, Shams-Asanjan K, Movasaghpoor-Akbari A, Talebi M et al . The effect of resveratrol on the expression of MDR1 gene in leukemic lymphoblast’s of acute lymphoblastic leukemia patients. Caspian J Intern Med 2015; 6 (2) :113-115
URL: http://caspjim.com/article-1-427-en.html

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Volume 6, Issue 2 (4-2015) Back to browse issues page
Caspian Journal of Internal Medicine
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